Evaluation of New Propyl Gallate APTT to Factor Assay Sensitivity with Platelet Depleted Plasma

Analytical Control Systems, Inc

Analytical Control Systems, Inc.

info@analyticalcontrols.com

Phone ACS

317-841-0458

Fax ACS

317-841-3186

Mail ACS

Analytical Control Systems, Inc.

9058 Technology Drive

Fishers, IN 46038

Many of Analytical Control Systems, Inc. products are covered by US and foreign patents or patents pending.

Platelet Depleted Plasma, Spectra™ APTT and Factor Assay Sensitivity

Evaluation of New Propyl Gallate APTT to Factor Assay Sensitivity with Platelet Depleted Plasma

By R Speck, D Bonderman, C Speck and J Griep Nov 15, 1996.

Analytical Control Systems, Inc. has developed a new, patented highly sensitive Activated Partial Thromboplastin Time (APTT) assay. Increased sensitivity to factor assays have been established using this new APTT reagent with platelet depleted plasma (PDP). The following table shows data comparing the new propyl gallate (PG) APTT reagent with the ellagic acid (EA) APTT for Factors II, VIII, Fletcher and Fitzgerald assays using PDP. This assay also shows some increased sensitivity to factors V, IX, X, XI, and XII.

Propyl Gallate APTT Vs. Ellagic Acid APTT- Sensitivity to Factors II, VIII, Fletcher and Fitzgerald
%	Factor II		Factor VIII		Fletcher Factor		Fitzgerald Factor
Factor	PG (sec)	EA (sec)	PG (sec)	EA (sec)	PG (sec)	EA (sec)	PG (sec)	EA (sec)
1	90.7	56	94	65	52	32	69	30
10	85	53.8	87.5	62	50	31.8	65.8	30
20	78.5	50.9	81.3	58.2	47.8	31.8	61.5	30
40	66	45.5	69	52	43.5	31	53.5	29.5
60	54	40	58	45.8	39	31	45.5	29.4
80	54.5	35	46	39	35	30.4	36.8	29
100	29	29	34	32	30	30	28	28

Particularly important was the discovery of this assay’s sensitivity to Fletcher and Fitzgerald factors since they could not be assayed by other APTT reagents. Below is a plot comparing the sensitivity of the two APTT reagents to Fitzgerald Factor.

Propyl Gallate APTT Vs. Ellagic Acid APTT Sensitivity to Fitzgerald Factor

wpe16.jpg (10346 bytes)

Return to APTT Research Index