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Release of Platelet Factor 3 Activity was Determined in Single Platelet Concentrate Units by a New Procedure and the Results Reported

Abstract by A Thelia, D. Gao, D. Bonderman, R. Speck and J. Griep, Central Indiana Regional Blood Bank, Indianapolis, IN. Nov., 15, 1996.

Propyl gallate is a new, patented chemical activator which rapidly releases platelet factor 3 (PF3) and activates the factors in the intrinsic plasma coagulation pathway. This reagent was used to measure the release of platelet factor 3 in single platelet concentrate units using the activated plasma clotting time (APCT) procedure. Platelets were diluted with platelet depleted plasma (PDP) and the APCT test performed on each dilution. The maximum dilution was determined which would support clotting. Platelet counts were also performed on each unit.

There was no correlation between the release of PF3 activity of the single platelet concentrate unit and the platelet count nor the age of the unit. This initial study shows the variability of the release of PF3 activity in single platelet concentration units. The release of PF3 activity of the single platelet concentration unit may be an important criterion for selecting units to be used in platelet therapy.